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Can be used for legal purposes, but it must meet certain requirements. In cases of legal paternity testing, a DNA test can be done without the mother's participation. However, the test must be conducted under strict legal standards to ensure its admissibility in court.

Key conditions for legal use of a motherless DNA test:

1. Chain of Custody: The DNA samples must be collected following a documented and witnessed procedure. This ensures that the samples are properly identified and handled.
2. Accredited Lab: The DNA test should be conducted at a laboratory that is accredited by relevant authorities, such as the AABB (American Association of Blood Banks) in the U.S. or similar organizations in other countries.
3. Court Approval: If the test is intended to be used in a legal case (e.g., for child support, custody, or inheritance), it should meet the specific legal requirements of the court where the case is being heard.
4. Witnessed Sample Collection: The collection process must be witnessed, often by a third party like a healthcare provider or legal representative, to ensure the validity of the test.